Figure 2
Cat S deficiency inhibits tumor growth and metastasis. (A) Representative images of tumors of WT and Cat S-/- mice 28 days after subcutaneously inoculation of PancO2 cells. A suspension of 1 × 106 PancO2 cells was injected subcutaneously into the flank of anaesthetized WT and Cat S-/- mice. Mice were killed 28 days after transplant, and tumors were weighted after dissection. Bar = 1 mm. Data are mean ± SEM for n = 10 mice **, P<0.01. (B) Immunohistochemical analysis of Ki-67 expression in PancO2 tumor burden into WT and Cat S-/- mice. (×400 magnification and Scale bars = 50 μm). Quantitative analysis of Ki-67 positive cells in tumor sections. Data are mean ± SEM for n = 10 mice with 10 fields per animal. **, P<0.01 vs WT mice. (C) Gross examination of development of liver metastasized tumor of colon cancer after intrasplenic injection of SL4 cells in WT and Cat S-/- mice. SL4 cells (1 × 106) were injected into the spleen of WT and Cat S-/- mice. Mice were sacrificed at 14 days after tumor injection to determine the incidence of liver metastasis and tumor weight. The normal livers from WT or Cat S-/- mice as control groups. Data are mean ± SEM for n = 10 mice **, P<0.01. (D) The analysis of micrometastasis was performed on paraffin-embedded sections with HE staining in metastasized foci after intrasplenic injection of SL4 cells in WT and Cat S-/- mice. Number of micrometastatic lesions per one representative cross section of the livers from each mouse. (×100 magnification and Scale bars = 100 μm). Data are mean ± SEM for n = 10 mice with 10 fields per animal. **, P<0.01.