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Figure 3 | Molecular Cancer

Figure 3

From: Cathepsin S-mediated autophagic flux in tumor-associated macrophages accelerate tumor development by promoting M2 polarization

Figure 3

Cat S deficiency reduces M2 macrophage infiltration in tumor development. (A) Immunohistochemical analysis of macrophage infiltration in metastasized foci after intrasplenic injection of SL4 cells in WT and Cat S-/- mice. Macrophage infiltration detected by anti-Mac-2 immunostaining (×400 magnification and Scale bars = 50 μm). (B) Quantitative analysis of Mac-2 positive cells in metastasized foci sections. Data are mean ± SEM for n = 8 mice with 10 fields per animal. NS indicates not significant. (C) Double-color immunofluorescence analysis of macrophage and Cat S expression in metastasized foci from WT and Cat S-/- mice. The sections were immunostained with anti-F4/80 and anti-Cat S antibodies. (×400 magnification and Scale bars = 50 μm). (D) Double-color immunofluorescence analysis of M2 macrophage expression in metastatic foci from WT and Cat S-/- mice. The sections were immunostained using the combination of anti-F4/80 and anti-CD206 antibodies. (×400 magnification and Scale bars = 50 μm). (E) Leukocytes were gated with CD45 fluorescence, macrophage M2 marker (CD45+F4/80+CD206+) were detected by flow from metastatic foci in the liver after intrasplenic injection of SL4 cells in WT and Cat S-/- mice. Data are mean ± SEM for n = 8 mice per group. NS indicates not significant. **, P<0.01.

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