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Table 1 Primers used for qRT-PCR

From: Cathepsin S-mediated autophagic flux in tumor-associated macrophages accelerate tumor development by promoting M2 polarization

Primer

Forward

Reverse

Human cathepsin B

5′gtttgcattgctggtcagga3′

5′tggcaggacagtggaatgat3′

Human cathepsin D

5′gcgagtacatgatcccctgt3′

5′ctctggggacagcttgtagc3′

Human cathepsin F

5′ tggcaacaagatgaagcaag3′

5′ttttgtgacagcccccttac3′

Human cathepsin H

5′actggctgttgggtatggag3′

5′ aggccacacatgttctttcc3′

Human cathepsin K

5′ccgcagtaatgacacccttt3′

5′gcacccacagagctaaaagc3′

Human cathepsin L

5′acagtggaccaagtggaagg3′

5′cttctcccacactgctctcc3′

Human cathepsin S

5′tcatacgatctgggcatgaa3′

5′aggttctgggcactgagaga3′

Human cathepsin Z

5′aagggggtaatgacctgtcc3′

5′ttcattgcatgtcccacatt3′

Human GAPDH

5′acagtcagccgcatcttctt3′

5′acgaccaaatccgttgactc3′

Mouse Arg-1

5′aaagctggtctgctggaaaa3′

5′ acagaccgtgggttcttcac3′

Mouse FIZZ1

5′ttgcaactgcctgtgcttac3′

5′ctgggttctccacctcttca3′

Mouse IL-10

5′ccaagccttatcggaaatga3′

5′ttttcacaggggagaaatcg3′

Mouse iNOS

5′gggctgtcacggagatca3′

5′ccatgatggtcacattctgc3′

Mouse IL-1β

5′gcccatcctctgtgactcat3′

5′aggccacaggtattttgtcg3′

Mouse TNF-α

5′tcttctcattcctgcttgtgg3′

5′ggtctgggccatagaactga3′

Mouse Tubulin

5′tctaacccgttgctatcatgc3′

5′gccatgttccaggcagtag3′

  1. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Arg-1, arginase-1; FIZZ1, found in inflammatory zone 1; IL-10, interleukin 10; iNOS, inducible NO synthase; IL-1β, interleukin 1β; TNF-α, tumor necrosis factor-alpha.