Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Chemoprevention of dietary digitoflavone on colitis-associated colon tumorigenesis through inducing Nrf2 signaling pathway and inhibition of inflammation

Figure 3

Effects of digitoflavone on oxidative stress-induced cell injury in Caco-2 cells. (A) The anti-oxidative ability of digitoflavone. Caco-2 cells were pretreated with 10 μM digitoflavone for 4 hours then espoused to various concentration of H2O2 for an additional 24 h. Cell viability was determined using MTT assay. (B) Dose-dependent anti-oxidative ability of digitoflavone. Caco-2 cells were pretreated with 1–15 μM digitoflavone for 4 hours then espoused to 500 μM H2O2 for an additional 24 h. Cell viability was determined using MTT assay. (C) Nrf2 silencing partly abrogates digitoflavone-induced cytoprotective effect. After 48 h transfection, the cells were treated with 10 μM digitoflavone for 4 hours and followed an oxidative stress of 500 μM H2O2 for 24 hours, Cell viability was determined using MTT assay. (D) GSH synthetise inhibitor-BSO partly abrogates digitoflavone-induced cytoprotective effect. Caco-2 cells were pretreated with 10 μM BSO for 2 hours, then cells were treated with 10 μM digitoflavone for an additional 4 hours, anti-oxidative ability was tested with 500 μM H2O2 for an additional 24 h using MTT assay. (E) Flow cytometry analysis of the intracellular ROS. Caco-2 cells were treated with various concentrations of digitoflavone for 4 hours before espoused to 500 μM H2O2 for an additional 4 h. Intracellular ROS levels were measured using DCF fluorescence. (F) Statistical analysis of the flow cytometry date. (G) Flow cytometry analysis of the apoptotic rate. Caco-2 cells were treated with various concentrations of digitoflavone for 4 hours before espoused to 500 μM H2O2 for an additional 6 h. cells were stained with FITC-Annexin V-PI, flow cytometry measured the apoptotic rate. (H) Statistical analysis of the apoptotic rate. The values represent the means ± SD of triplicate experiments. Significantly different: *p<0.05, **p<0.01.

Back to article page