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Figure 4 | Molecular Cancer

Figure 4

From: Chemoprevention of dietary digitoflavone on colitis-associated colon tumorigenesis through inducing Nrf2 signaling pathway and inhibition of inflammation

Figure 4

Effects of signaling inhibitors on digitoflavone-induced Nrf2 and γ-GCSc expression in Caco-2 cells. (A) Possible mechanism of digitoflavone-induced Nrf2 activation. Caco-2 cells were treated with 10 μM digitoflavone for 0, 1, 2, 4, 8 hours, Keap1 expression level and Nrf2 upstream kinases were determined using Western blotting and appropriate specific antibodies. (B) Role of Erk1/2 and PI3K/AKT in digitoflavone-induced Nrf2 activation. Caco-2 cells were pretreated with various concentration of PD98059 (MEK1/2 inhibitor), wortmannin (for PI3K inhibitor) for 2 hours respectively, then cells were treated with 10 μM digitoflavone for an additional 8 hours, Nrf2 and γ-GCSc expression were determined using Western blotting. (C-D) Caco-2 cells were pretreated with 10 μM PD98059 or 10 μM wortmannin for 2 hours respectively, then cells were treated with 10 μM digitoflavone for an additional 4 hours, anti-oxidative ability was tested with 500 μM H2O2 for an additional 24 h using MTT assay. The values represent the means ± SD of triplicate experiments. Significantly different: *p<0.05, **p<0.01.

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Molecular Cancer

ISSN: 1476-4598