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Figure 3 | Molecular Cancer

Figure 3

From: Reduced expression of AMPK-β1 during tumor progression enhances the oncogenic capacity of advanced ovarian cancer

Figure 3

Depletion of AMPK-β1 promotes cell proliferation and anchorage-independent growth ability. (A) XTT cell proliferation assay demonstrating that OV2008 (C2, C5 and C32) (P < 0.002) and OVCA433 (C1, C12 and C23) (P < 0.001) clones exhibited a 20-25% reduction in cell proliferation. (B) Focus formation assay showing that all AMPK-β1 stable knockdown clones in A2780cp (C2 and C5) (P < 0.001) and OVCA433 (C1 and C12) (P < 0.001) cells expressed a 2 to 3-fold increase in the number of foci. (C) Soft agar assay showing that knockdown of AMPK-β1 in OV2008 (C2 and C5) (P < 0.03) and OVCA433 (C1 and C12) (P < 0.03) cells had a 4-5-fold increase in the number and size of colonies. P: parental. V, V1 or V2: empty vector controls.

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Molecular Cancer

ISSN: 1476-4598