Expression of CTCF in leukemic cells before and after treatment with an inhibitor of NF-κB activity. (A) Nalm-6 cells were treated with the NF-κB inhibitor PDTC or DMSO (negative control) for 20 h. NF-κB pathway activity was detected by Western blot using a nuclear p65-specific antibody. Histone H3 and α-tubulin were used as the loading controls for nuclear and cytoplasmic proteins, respectively. (B) CTCF mRNA levels in cell lysates were measured by real-time PCR. The relative changes in the expression levels were analyzed and presented as mean ± SD from triple replications (fold change 2.49, p = 0.000). (C) Cell lysates were probed with an anti-CTCF antibody, and the expression levels of CTCF were semi-quantified by analyzing the Western blot with Gel-Pro Analyzer software (fold change 1.89). GAPDH was used as a loading control.