Figure 3

Treatment with SW IV-134 results in caspase-mediated cell death. (A) Cells were treated with vehicle only (Ctrl.), SW43 (10 μM), and SW IV-134 (10 μM) for 24 hours. Cell lysates were subjected to Western blot analysis, and probed with antibodies specific for the precursors and activated forms of caspases 8, 9, and 3. (B) SKOV3 cells were treated with SW IV-134 at indicated doses either alone or in the presence of the pan-caspase inhibitor Z-VAD-FMK (20 μM) for 18 hours. Cell viability was determined by CellTiter-Glo assays (p < 0.018, Student’s t-test).