Figure 5
From: Conjugation to a SMAC mimetic potentiates sigma-2 ligand induced tumor cell death in ovarian cancer
SW IV-134 induces activation of the TNF α -dependent extrinsic death pathway. (A) Quantitative real-time PCR analysis of TNFα mRNA expression was done on RNA samples derived from SKOV3 cells treated with SW IV-134 (1 μM and 3 μM) for indicated time periods. All values were normalized to a GAPDH internal control (p < 0.0001, one-way ANOVA). (B) SKOV3 cells were treated with SW IV-134 (1 μM and 3 μM) and cell-culture supernatants were collected at 4, 12, and 24 hours after treatment, and subsequently examined by anti-TNFα ELISA according to manufacturer’s instructions (p < 0.0001, one-way ANOVA). (C) SKOV3 cells were treated with SW IV-134 at indicated doses either alone or in the presence of anti-TNFα blocking antibody for 18 hours. Cell viability was determined as described in the experimental methods (p < 0.019, Student’s t-test).