Figure 2
From: TGFβR2 is a major target of miR-93 in nasopharyngeal carcinoma aggressiveness
miR-93 directly suppresses TGFβR2 in NPC. (A) miRNA expression profiling analysis in three subgroups of clinical samples including 8 NP with high TGFβR2 expression (H-NP, a blue bar on top), 7 NPC with high TGFβR2 expression (H-NPC, a brown bar on top) and 7 NPC with low TGFβR2 expression (L-NPC, a red bar on top). (B) qRT-PCR validation of miR-93 expression in 22 clinical tissue samples. Values represent mean ± SD, n = 3. **P < 0.01. ***P < 0.001. (C) Correlation analysis between TGFβR2 and miR-93 in 22 clinical tissue samples. (D) RNAhybrid prediction of the binding sites of miR-93 in the 3′ UTR of TGFβR2. Luciferase activities of dual luciferase reporter carrying a wild-type or mutant of TGFβR2 3′ UTR in the indicated cells in triplicate. Values represent mean ± SD. *P < 0.05. (E, F) The detection of TGFβR2 mRNA and protein expressions by qRT-PCR and Western blot in CNE1 and CNE2 cells transfected with miR-93 mimic and inhibitor respectively. Values represent mean ± SD, n = 3. **P < 0.01.