Regulation of Smad-dependent TGF-β signaling, PI3K/Akt signaling, cell cycle progression and EMT by miR-93-mediated TGFβR2 inactivation in NPC cells. (A) Western blotting analyses of Smad2/3 and its phosphorylation as well as PI3K and p-Akt in the indicated cell groups. (B) Western blotting analyses of C-myc, E2F1, cyclin D1, CDK4 and p21 in the indicated cell groups. (C) Western blotting analyses of Snail, E-cadherin, and Vimentin in the indicated cell groups. (D) The morphological observations of the indicated cell lines. CNE1 cells were transfected with miR-93 mimic/siRNA-TGFβR2 and miRNA/siRNA control for 96 hours and analyzed under phase contrast microscopy (400×).