Figure 4From: Hyaluronan-CD44 interaction promotes c-Jun signaling and miRNA21 expression leading to Bcl-2 expression and chemoresistance in breast cancer cellsDetection of HA/CD44-induced miR-21 production in MDA-MB-468 cells. Detection of miR-21 in MDA-MB-468 cells using RNase protection assay as described in Materials and methods. a: Autoradiogram of miR-21 detected in MDA-MB-468 cells incubated without HA (a: lane 1) or with 2 h HA treatment (a: lane 2) or pretreated with anti-CD44 antibody for 1 h followed by HA addition for 2 h (a: lane 3) or incubated with scrambled siRNA plus 2 h HA treatment (a: lane 4) or c-Jun siRNA plus 2 h HA treatment (a: lane 5) or incubated with negative miRNA control plus 2 h HA treatment (a: lane 6) or incubated with anti-miRNA-21 plus 2 h HA treatment (a: lane 7) or 2 hours HA treatment (a: lane 8) or pretreated cells with JNK Inhibitor (420116) plus 2 h HA treatment (a: lane 9) or treated with non-immune IgG (without HA addition) (lane 10) or treated with non-immune IgG (with HA addition for 2 h). [Autoradiogram of miR-191 (b) in each gel lane was used as a loading control]. [The ratio of miRNA-21 (a) and miRNA-191 (the loading control) (b) was determined by densitometry, and the levels were normalized to untreated (without HA treatment) value or non-immune IgG (without HA treatment) (designated as 1.00); the values expressed represent an average of triplicate determination of 4 experiments with an SD of less than 5%].Back to article page