Hyaluronan-CD44 interaction promotes c-Jun signaling and miRNA21 expression leading to Bcl-2 expression and chemoresistance in breast cancer cells

Table 1 Measurement of HA-induced p-c-Jun and c-Jun nuclear accumulation in MDA-MB-468 cells

Treatments	p-c-Jun nuclear accumulation (% of control)	c-Jun nuclear accumulation (% of control)
No HA (control)	100% ± 5	100% ± 3
+ HA	275% ± 13^a	260% ± 10^b
Non-immune IgG (No HA)	102% ± 4^a	101% ± 5^b
Non-immune IgG (+HA)	268% ± 9^a	275% ± 11^b
Anti-CD44 antibody + HA	92% ± 3^a	87% ± 3^b
Vehicle control + HA	262% ± 8^a	279% ± 8^b
JNK inhibitor + HA	84% ± 4^a	75% ± 3^b

p-c-Jun or c-Jun was detected in the nucleus which was stained with Texas Red-labeled anti-p-c-Jun antibody or FITC-labeled anti-c-Jun antibody (as shown in Figure 2). The labeling index for p-c-Jun or c-Jun was indeoendently determined by two observers and corresponded to the percentage of positive nuclear accumulatiohjn among 1,000 cells using Epi-fluorescence microscope (at 40X objectives).
All data represent mean ± SEM (with n = 4) of p-c-Jun and c-Jun nuclear accumulation activity detected in each sample.
^{a, b}Statistically significant (P < 0.005; analysis of variance; n = 4) as compared with control samples (No HA treatment).

ISSN: 1476-4598