Treatments | Apoptotic cells (Annexin V-positive cell/total cells × 100%)* | Doxorubicin-induced tumor cell growth inhibition IC50(μM)** | |
---|---|---|---|
No doxorubicin | + doxorubicin | ||
(A) Effects of various signaling perturbation agents on doxorubicin--induced apoptosis and cell growth inhibition in MDA-MB-468 cells. | |||
(a) Effects of Doxorubicin-induced apoptosis and cell growth inhibition in MDA-MB-468 cells following HA treatment: | |||
No HA (Untreated cells) | 4.1 ± 0.5 | 30.2 ± 3.1a | 73.5 ± 5.1 |
+ HA | 2.4 ± 0.3a | 12.6 ± 2.3a | 188.0 ± 10.5b |
Non-immune rat IgG-treated cells (No HA) | 3.8 ± 0.5a | 29.2 ± 2.2a | 70.3 ± 4.1b |
Non-immune rat IgG-treated cells (+ HA) | 2.2 ± 0.2a | 10.5 ± 2.0a | 176.0 ± 10.3b |
Anti-CD44-treated cells (+ HA) | 3.8 ± 0.2a | 29.2 ± 2.2a | 28.4 ± 3.6b |
(b) Effects of Doxorubicin-induced apoptosis and cell growth inhibition in MDA-MB-468 cells treated with c-Jun siRNA plus HA: | |||
Scrambled siRNA-treated cells (No HA) | 5.2 ± 0.6 | 32.4 ± 3.6c | 85.0 ± 2.4 |
Scrambled siRNA-treated cells (+ HA) | 4.5 ± 0.5c | 11.9 ± 3.1c | 190.0 ± 6.00d |
c-Jun siRNA-treated cells (No HA) | 5.2 ± 0.6c | 33.6 ± 2.8c | 40.5 ± 0.30d |
c-Jun siRNA-treated cells (+ HA) | 4.5 ± 0.5c | 31.9 ± 3.2c | 39.2 ± 0.80d |
(c) Effects of Doxorubicin-induced apoptosis and cell growth inhibition in MDA-MB-468 cells treated with anti-miR-21 inhibitor plus HA: | |||
miRNA negative control-treated cells (No HA) | 3.7 ± 0.4 | 31.6 ± 2.9e | 62.8 ± 0.5 |
miRNA negative control-treated cells (+ HA) | 2.5 ± 0.2e | 12.3 ± 0.4e | 196.0 ± 4.2f |
Anti-miR-21 inhibitor-treated cells (No HA) | 3.2 ± 0.6e | 38 ± 2.5e | 65.0 ± 3.0f |
Anti-miR-21 inhibitor-treated cells (+ HA) | 3.1 ± 0.4e | 34 ± 2.1e | 70.0 ± 0.2f |
(B) Effects of JNK inhibitor (420116) on Doxorubicin-induced apoptosis and cell growth inhibition in MDA-MB-468 cells. | |||
Vehicle control-treated cells (No HA) | 4.3 ± 0.2 | 32 ± 2.4g | 64.00 ± 6.2 |
Vehicle control-treated cells (+ HA) | 2.6 ± 0.4g | 14 ± 0.5g | 180.0 ± 10.0h |
JNK inhibitor (420116)-treated cells (No HA) | 12.0 ± 0.5g | 35 ± 2.6g | 26.0 ± 3.5h |
JNK inhibitor (420116)-treated cells (+ HA) | 3.2 ± 0.3g | 38 ± 3.3g | 25.0 ± 0.2h |