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Figure 2 | Molecular Cancer

Figure 2

From: Gold (I) N-heterocyclic carbene complex inhibits mouse melanoma growth by p53 upregulation

Figure 2

Growth inhibition and the subsequent evaluation of apoptosis of cancer cells in presence of complex 3. A, Percentage growth inhibition of HCT 116, HepG2, A549 and B16F10, following treatment with complex 3 (0, 2.5, 5,10 and 25 μM) for 24 h. B, Percentage of growth inhibition of HCT-116, HepG2, A549 and B16F10, following treatment with GI50 concentration of complex 3 for 0, 12, 24 and 48 h. C, Percentage of growth inhibition of HCT-116, HepG2, A549 and B16F10, following treatment with cisplatin (0, 2.5, 5,10 and 25 μM) for 24 h. D, Morphological images of the B16F10 cells treated with complex 3 (0 and 10 μM) in the upper row. Acridine orange/EtBr stained images of B16F10 cells treated with complex 3 (0 and 10 μM) in the middle row. DAPI stained image of the B16F10 cells treated with complex 3 (0 and 10 μM) in the lower row. Scale Bar = 10 mm. Magnification at 40×. E, Flow cytometric analysis of apoptosis induction in B16F10 cells treated with complex 3 (0 and 10 μM) for 24 h. F, Cell cycle analysis of complex 3 (0 and 10 μM) treated B16F10 cells for 24 h. G, DNA fragmentation analysis of complex 3 (0, 2.5, 5, 10 and 25 μM) treated B16F10 cells for 24 h. Values are mean ± S.D and represent one of the 3 representative experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.

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