Figure 2

ETS expression and RAS activation induce migration of prostate cells via the same pathway. (A) A transwell assay measured relative number of migrating RWPE cells expressing ERG or activated KRAS relative to normal RWPE cells (first lane). (B, C) Transwell assays measured migration of (B) RWPE cells, or (C) RWPE-KRAS cells expressing oncogenic (Black bars) or non-oncogenic (Grey bars) ETS proteins. Number of cells migrated is reported relative to the same cell line transduced with an empty vector (white bar). Mean and SEM of three biological replicates (each mean of two technical replicates) are shown for (A) and five biological replicates for (B) and (C). P-values compare indicated value to the hypothetical mean (1) and are calculated by t test: * < 0.05, ** < 0.005, unmarked > 0.05.