PI3K/AKT signaling in oncogenic ETS function is not through mTORC1. (A) shRNA knock down of mTOR, Raptor (mTORC1 complex) and Rictor (mTORC2 complex) in RWPE-ERG cells was confirmed by qRT-PCR analysis. Mean and SEM of two biological replicates (each mean of two technical replicates) are shown. (B) A transwell assay measured cell migration of RWPE-ERG cells stably expressing the indicated shRNA relative to a negative control (shRNA targeting luciferase, which is not expressed in this cell line). Results are the mean and SEM of four independent experiments, each the mean of two technical replicates. (C) Immunoblot showing the expression level of pAKT and tubulin in RWPE-ERG cells expressing the indicated shRNA. P-values are calculated by t test: *** < 0.0005.