Figure 1
From: Thioredoxin interacting protein (TXNIP) is a novel tumor suppressor in thyroid cancer
TXNIP is highly expressed in DTC cell lines and low or undetectable in ATC cell lines, and glucose uptake is inversely proportional to TXNIP expression levels. (A) HTh74 cells were transduced with lentivirus expressing a PPARĪ³-specific shRNA or scrambled control, and stable pools were generated under antibiotic selection. Western blot analysis of nuclear lysates (top immunoblot) reveals PPARĪ³ expression levels in the transduced cells. Using Western blot analysis of whole cell lysates, TXNIP and Ī²-actin (loading control) were detected using specific antibodies. Nonspecific band is indicated by ānsā. (B) Western blot analysis for TXNIP, Trx-1, and Ī²-actin (loading control) were performed on whole cell lysates prepared from a panel of DTC and ATC cell lines grown under standard conditions. (C) Glucose uptake assays were performed. Each condition was performed in triplicate per experiment, and each experiment was performed at least three times. Nonspecific glucose uptake as determined by parallel treatment of a subset with cytochalasin B was subtracted from measurements. Data from all experiments were combined, and glucose uptake from each cell line was normalized to levels of HTh74 (average set at 1). Normalized averages are plotted, and error bars indicate SEM. ***p <0.0001, **p <0.001.