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Figure 4 | Molecular Cancer

Figure 4

From: Thioredoxin interacting protein (TXNIP) is a novel tumor suppressor in thyroid cancer

Figure 4

TXNIP overexpression inhibits in vitro growth of ATC HTh74 cells. Viable cell proliferation assays were performed for stable HTh74 (A) and T238 (B) cell lines. Briefly, 50,000 cells were plated in 6-cm plates, trypsinized and viable cell counts were determined using the ViCell automated cell counting system. Each time point was performed in duplicate, and lysates were prepared from the day 7 time point to confirm high TXNIP expression using Western blot analysis. Each experiment was performed at least three times. Mean and SEM are plotted. Closed squares (āˆŽ) indicate vector control cells, and open diamonds (ā—Š) indicate cells with TXNIP overexpression. In vitro invasion assays were performed on the TXNIP-overexpressing stable HTh74 (C) and T238 (D) cell lines as described in the Methods section. Results from three independent experiments were combined and normalized to the vector control average and graphed with mean plus SEM. *p <0.01 (Figure 4A), Figure 4B pā€‰=ā€‰0.9021, Figure 4C pā€‰=ā€‰0.3523, Figure 4D pā€‰=ā€‰0.0754.

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