Mutations in CEACAM1 promoter fail to ablate GFP expression when fused to CEACAM1 6-7-8 minigene. (A) Schematic diagram of CEACAM1’s WT promoter and mutants A- > T and GG->CC (upper black arrow) were translationally fused to exons 6-7-8 minigene. Only AS to generate the L-isoform expresses the GFP reporter. A mCherry reporter that was co-transfected and used as a quantitation control was fused to CEACAM1 WT ISRE (lower black arrow). (B-C) Flow cytometric analyses, histogram and statistical analyses of Q2 in Figure 4B were similar to description in Figure 2 legend.