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Figure 4 | Molecular Cancer

Figure 4

From: IRF-1 regulates alternative mRNA splicing of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) in breast epithelial cells generating an immunoreceptor tyrosine-based inhibition motif (ITIM) containing isoform

Figure 4

Mutations in CEACAM1 promoter fail to ablate GFP expression when fused to CEACAM1 6-7-8 minigene. (A) Schematic diagram of CEACAM1’s WT promoter and mutants A- > T and GG->CC (upper black arrow) were translationally fused to exons 6-7-8 minigene. Only AS to generate the L-isoform expresses the GFP reporter. A mCherry reporter that was co-transfected and used as a quantitation control was fused to CEACAM1 WT ISRE (lower black arrow). (B-C) Flow cytometric analyses, histogram and statistical analyses of Q2 in Figure 4B were similar to description in Figure 2 legend.

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Molecular Cancer

ISSN: 1476-4598