RNA SEQ of Ad-IRF-1 treated breast carcinoma cells, MDA-MB-468. (A) RNA SEQ Workflow. Reads were analyzed for exclusion, inclusion and differential gene expression in -/+ induction by Ad-IRF-1 after 24 h. Only genes with high inclusion but low expression reads were considered candidates for further study and validated by exon junction specific PCR. (B) A Venn diagram summarizing the overlap between genes differentially expressed. Exons exclusively expressed (representing exon inclusion; red circle), exons dowregulated (yellow circle) are shown in this array. (C) Quantitative RT-PCR of candidates as compared to control sample CEACAM1. Primer pairs were designed as exon junction to recognize upstream and variable exon with priming on the downstream exon as shown above the figure.