Expression of ENO1 gene in glioma and NB tissues. (A). mRNA expression of ENO1 is decreased in NB tissues compared with glioma tissues by real-time PCR assay. Data are presented were presented as mean ± SD for three independent experiments (*P < 0.05). (B). Western blot analysis of ENO1 expression in 10 cases of WHO IV glioma tissue samples compared with 4 NB tissues. The unpaired t-test was used for this assay (*P < 0.05). (C). ENO1 expression was increased in 136 primary glioma samples compared to 15 normal brain tissues using immunohistochemical staining. a). Weak staining of ENO1 in NB tissues. b). Strong staining of ENO1 in NB tissues. c and d). Weak staining of ENO1 in glioma samples. e and f). Strong staining of ENO1 in glioma samples. Original magnification 400 × .