Figure 3From: Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in gliomaEffect of shRNA to stably knock down the expression of ENO1 in human glioma cell lines U251 and U87. Different treatments included PLV-Ctr. (A). RT-PCR shows transcriptional levels of the ENO1 gene with ARF used as a loading control. (B). Western blot showing protein expression levels in shENO1 and PLV-Ctr treatments. A representative image of three different experiments is shown. β-actin served as a loading control. Bar graph shows the relative expression of protein among the groups. Data are presented as mean ± SD for three independent experiments (*P < 0.05, **P > 0.05).Back to article page