Stably inhibited ENO1 expression decreases cell migration and invasion. (A). Stably downregulating ENO reduced the migration ability of shENO1-U251 and shENO1-U87 cells in vitro. (B). Stably suppressed ENO1 reduced in vitro invasion of shENO1-U251 and shENO1-U87 cells. (C). Transiently downregulated ENO1 dramatically decreased the migration ability of U251 and U87 cells in vitro. (D). Transiently suppressed ENO1 inhibited in vitro invasion of U251 and U87 cells. Data were presented were presented as mean ± SD for three independent experiments. *P < 0.05, statistically significant difference.