Figure 4From: Inhibition of STAT3 dimerization and acetylation by garcinol suppresses the growth of human hepatocellular carcinoma in vitro and in vivoGarcinol reduces cellular proliferation and induces apoptosis in HCC cells. (A) Garcinol inhibits proliferation of HCC cells in a dose and time dependent manner. Different cell lines were plated in triplicate, treated with indicated concentrations of garcinol, and then subjected to MTT assay after 24, 48 and 72 h to analyze proliferation of cells. Data represents result of three independent experiments. (B) Garcinol promotes apoptosis: C3A cells were treated with 50 μM garcinol and the whole cell extracts were subjected to immune-blotting with antibodies against pro-caspase-3, cleaved caspase-3 and PARP taking β-actin as loading control. (C) Garcinol potentiates the apoptotic effect of doxorubicin and paclitaxel. C3A cells (1×106/ml) were treated with 10 μM garcinol and 10 nM doxorubicin or 5 nM paclitaxel alone or in combination for 24 h at 37°C. Cells were stained with a Live/Dead assay reagent for 30 min and then analyzed under a fluorescence microscope as described in Materials and Methods.Back to article page