Garcinol inhibits the growth of human HCC in vivo. (A) Garcinol treatment reduced the size of tumor induced in nude mice. Athymic mice bearing subcutaneous PLC/PRF5 tumors were treated for 5 times a week for 4 consecutive weeks with 1 mg/kg and 2 mg/kg garcinol (n = 5) or corn oil alone (n = 4). Tumor size was measured and compared for different conditions. (B) Reduction in tumor volume upon garcinol treatment was statistically significant. * = P < 0.001 (One-way ANOVA). (C) Immunohistochemical analysis showed the inhibition in expression of phospho-STAT3, and Bcl-2 and increased levels of caspase-3 expression in garcinol treated mice tissues as compared with control group. Percentage indicates positive staining for the given biomarker. The photographs were taken at the magnification of × 20.