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Figure 2 | Molecular Cancer

Figure 2

From: RETRACTED ARTICLE: Exemestane blocks mesothelioma growth through downregulation of cAMP, pCREB and CD44 implicating new treatment option in patients affected by this disease

Figure 2

Exemestane acts in MPM cells by cAMP and CD44. A, cAMP ELISA detects a decrease in cAMP levels upon 35 μM exemestane treatment for 30 min (EXE), compared to untreated (CNTR), in all lines except NCI. B, western blot identifies CREB phosphorylation (pCREB) inhibition in cell lines responsive to exemestane treatment (E30) versus untreated (C30). C, flow cytometric characterizzazion of CD44 expression in untreated and treated MPM cell lines. Data are presented as a count of positive cells and numerical value indicates CD44 mean fluorescence intensity (MIF) of total cell population in the sample analyzed. Excluding NCI, all MPM cell lines show a reduction of MIF. D, siRNACD44 in MSTO and NCI reduced MPM cell lines migration in wound healing assay E, siRNA CD44 transfection resulted in decreased cell viability in MSTO and NCI cell lines compared to no CD44 siRNA. In addition, exemestane treatment further reduces the MSTO cell survival. No effect was observed in NCI. F, Western blot shows the silencing of CD44 occurring in MSTO and NCI. Moreover, the effect of exemestane in MSTO resulted in a decreased phosphorylation of AKT and CREB and in the reduction of Bcl-xL both in silenced cells that did not. No change was observed in NCI upon exemestane treatment both in silenced cells that did not. Graphs represent the average of at least 3 repeated experiments; *, statistically significant effects (P < 0.05).

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Molecular Cancer

ISSN: 1476-4598