Figure 3
From: Autophagic flux determines cell death and survival in response to Apo2L/TRAIL (dulanermin)
The autophagic flux is higher in C4-2 compared to PC3 cells. (A-C) Representative confocal time lapse images every 7 min and quantitation of green and red fluorescence for LC3B expression using GFP-mCherry-LC3 fusion protein stably expressed in PC3 and C4-2 cells following TR. (D-F) representative confocal images of fixed cells stably expressing GFP-mCherry-LC3 and quantitation of autophagosomal/autolysosomal LC3B-II puncta in PC3 and C4-2 cells following TR ± CQ. Yellow shows co-localization of GFP and mCherry, indicating completed autophagosomes (*p < 0.001). Data represent means ± SEM. (G) Representative confocal images of fixed cells for p62 (green) immunostaining following 8 h TR in PC3 and C4-2 cells. Nuclei were stained with DAPI.