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Figure 3 | Molecular Cancer

Figure 3

From: Integrated genomic analysis identifies the mitotic checkpoint kinase WEE1 as a novel therapeutic target in medulloblastoma

Figure 3

siRNA-mediated inhibition of WEE1 decreases medulloblastoma cell proliferation. (A) Daoy and UW228 cells transfected with a siRNA targeting WEE1 (siWEE1) displayed a decrease in cell proliferation when compared to a non-silencing siRNA (siRNA N.C.). Forty-eight hours after transfection cells were seeded into E-plates and real-time cell proliferation monitored using the xCELLigence system. (B) Transfection with siWEE1 significantly decreased the relative colony number compared to siRNA N.C. Representative pictures for each treatment group are above the quantifying bar graphs. (C) RNAi-mediated inhibition of WEE1 decreased the levels of WEE1 protein in both the Daoy and UW228 cell lines 72 hours after transfection.

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Molecular Cancer

ISSN: 1476-4598