Figure 1From: Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progressionModulation of HES1 and HEY1 expression by Notch signaling and PTOV1 in prostate cell lines. (A) The transcription of HES1 and HEY1 genes in RWPE1 and PC-3 cells is modulated by the γ-secretase inhibitor DAPT. Cells were treated with DAPT or solvent (CTL) for 4 days and HES1 or HEY1 transcript levels quantified by real-time RT-PCR. Right: The HES1 promoter is modulated by DAPT and by the negative dominant-MAML1 (dnMAML1). PC-3 cells, transfected with HES- luciferase and TK-Renilla, were either treated with DAPT or co-transfected with ICN and ICN plus dnMAML1 and firefly luciferase activity, normalized to Renilla, determined. (B) Knockdown of PTOV1 in RWPE and PC-3 prostate cells by shRNAs causes the upregulation of HES1 and HEY1 mRNA. Cells were transduced with shRNA1397 and shRNA1439 lentiviruses and analyzed by real-time RT-PCR. Values were normalized to RPS14 and for relative values in cells bearing control shRNA.Back to article page