Figure 3
Clonogenic and scratch-wound assays . (A) Clongenic growth of B78-H1 cells treated with porphyrin at concentration near the IC50: C14 (10 nM), P4 (250 nM). The cells have been treated with porphyrin /light and let to grow for one week. The colonies formed have been fixed, stained with methylene blue and counted. The number of colonies (>50 cells) is reported below each plate. The experiment has been perfomed in duplicate; (B) Scratch-wound assay of B78-H1 cells plated at density of 6 × 105 cells/well in a 6-well plate. After attachment, the cells were treated with 7.5 nM C14, 250 nM P2 and P4. A denudated area was created across the diameter of the dish with a yellow tip. Cells were washed with PBS and further incubated in complete medium. After light irradiation (7.2 J/cm2), pictures were taken by an epiluminescent microscope (at 10-fold magnification) to evaluate the migration of the cells. The experiment has been perfomed in duplicate.