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Figure 2 | Molecular Cancer

Figure 2

From: F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation

Figure 2

FBXL19 induces Rac3 degradation in the proteasome system - A. MLE12 cells were transfected with Rac3-V5 plasmid for 48 h. Cells were treated with MG-132 (20 μM) or leupeptin (100 μM) treatment for 1 h prior to change to blank medium for indicated incubation time. Cells lysates were analyzed by immunoblotting with V5 tag and β-actin antibodies. Rac3-V5 levels were quantified by Image J software. B. HEK293 cells were transfected with FBXL19-V5 plasmid for 48 h, and then the cells were treated with MG-132 (20 μM), Lactacystin (10 μM), leupeptin (100 μM), or NH4Cl (20 mM) for 4 h. Cells lysates were analyzed by immunoblotting with Rac3, V5 tag and β-actin antibodies. Rac3 levels were quantified by Image J software. C. MLE12 cells were co-transfected with Rac3-V5 and shFBXL19 plasmids for 48 h, and then the cells were cultured in blank medium (0–4 h). Cells lysates were analyzed by immunoblotting with V5 tag, FBXL19, and β-actin antibodies. Rac3-V5 levels were quantified by Image J software. All the blots are representative of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598