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Figure 5 | Molecular Cancer

Figure 5

From: F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation

Figure 5

Role of N-terminus and C-terminus of FBXL19 in Rac3 degradation - A. HEK293 cells were transfected with plasmid encoding wild-type FBXL19-V5 (Wt) or an FBXL19 variant with truncation at the NH2 (N111 or N121). Cell lysates were analyzed by immunoblotting with Rac3, V5 tag, and β-actin antibodies. B. HEK293 cells were transfected with plasmid encoding wild-type FBXL19-V5 (Wt) or an FBXL19 variant with truncation at the COOH (C450). Cell lysates were analyzed by immunoblotting with Rac3, V5 tag and β-actin antibodies. C. HEK293 cells were co-transfected with Rac3-Flag and FBXL19-V5 wild-type (Wt) or V5 tagged FBXL19 variant with truncation at the COOH (C450) plasmids. Cell lysates were subjected to immunoprecipitation with a Flag tag antibody or IgG, followed by V5 tag immunoblotting. Input lysates were analyzed by immunoblotting with V5 tag, Flag tag and β-actin antibodies. All the blots are representative of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598