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Figure 3 | Molecular Cancer

Figure 3

From: MicroRNA-155 as an inducer of apoptosis and cell differentiation in Acute Myeloid Leukaemia

Figure 3

miR-155 expression levels affect myeloid differentiation of AML cells. Expression of miR-155 48 hours after (A) ARAC induced apoptosis of OCI-AML3 cells or (B) VitD3 induced monocytic differentiation of OCI-AML3 cells. Data is presented as mean fold change expression of miR-155 + SEM relative to Untreated control; RNU6b was used as the reference gene, n = 3. VitD3 was used to induce myeloid differentiation in OCI-AML cells transfected with miR-155, anti-miR-155 or their respective controls (C) flow cytometry plots of phenotype analysis using CD11b and CD14 (D) miR-155 overexpression increased the percentage of CD11b + CD14+ cells at 48 hours (E) percentage expression of CD14 + CD11b + cells transfected with anti-miR155 and exposed to VitD3 (+) or PBS (-) for 48 hours (F) miR-155 overexpression increased the percentage of cells expressing CD15 at 48 hours without VitD3 stimulation (G) miR-155 inhibition decreased the percentage of cells expressing CD15. CTL- scrambled control; ARAC- cytarabine arabinoside; VitD3- 1,23-dihydroxyvitaminD3. Statistical significance determined using Paired Two Tailed t-Test, *p < 0.05; n = 4.

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