Figure 4
From: MicroRNA-155 as an inducer of apoptosis and cell differentiation in Acute Myeloid Leukaemia
Induction of apoptosis of OCI-AML3 cells after miR-155 transfection. (A) Gating strategy for flow cytometry analysis. Cells undergoing early apoptosis are stained for AnnexinV, cells undergoing late apoptosis are stained for both AnnexinV and 7-AAD (B) increase in AnnexinV positive cells (dashed line) or Double Positive cells for AnnexinV/7-AAD (solid line) post transfection with miR-155 (black line) compared to CTL (grey line) (C) summary of fold-change of cells (OCI-AML3, THP1, NB4, HL60, MV4-11) binding AnnexinV overexpressing miR-155 normalised to CTL at 24 and 48 hours post transfection. AnnV + AnnexinV positive cells; DP- double positive cells, CTL- Scrambled control. Statistical significance determined using Paired Two Tailed t-Test, *p < 0.05 of AnnV + cells; +p < 0.05 of DP cells, n = 4-7.