Figure 5
From: MicroRNA-155 as an inducer of apoptosis and cell differentiation in Acute Myeloid Leukaemia
Growth perturbations in AML cells with altered miR-155 expression levels. (A) Transfection of OCI-AML3 cells with miR-155 resulted in a reduction of cell growth at 24 h and 48 h as determined by MTS assay (B) Assessment of colony forming potential found a decrease in mean colony number and (C) a trend towards reduced colony size (presented as mean + SEM cells per colony) (D) Proliferation through BrdU incorporation did not demonstrate a significant change (E) Cell cycle analysis demonstrated an increase in the proportion of cells in the SubG1 fraction at 48 h post miR-155 overexpression. CTL, Scrambled Control (F) Transfection of OCI-AML3 cells with anti-miR155 LNA resulted in a significant decrease in the proportion of cells undergoing apoptosis (AnnexinV+) when treated with ARAC, but not when untreated. ARAC-cytarabine arabinoside; LNA-locked nucleic acid; CTL, Anti-Scrambled LNA. Statistical significance determined using Paired Two Tailed t-Test, *p < 0.05 ** p < 0.001; n = 3-4.