CD44 was a primary target of miR-34a in MIBC cells following cisplatin treatment. A) Relative CD44 expression in MIBC cells after cisplatin treatment relative to control group (cisplatin 0 μg/ml). B) CD44+ cells proportion decreased following miR-34a overexpression or cisplatin treatment. C) The seed regions of the miR-34a target sites in CD44 and the luciferase activity assay. D) Cisplatin-induced endogenous miR-34a upregulation caused the decrease of CD44-luciferase activity. E) CD44 protein expression was inhibited in miR-34a transfected MIBC cells. Data are plotted as the mean + SEM of 3 independent experiments. *p < 0.05.