Figure 3
From: Critical role of histone demethylase RBP2 in human gastric cancer angiogenesis
VEGF expression and angiogenesis in RBP2-targeted mutant mice. (A) QRT-PCR analysis of VEGF mRNA in the wild type (the number is 6), heterozygote (4) and mutant mice (3). **P < 0.01 vs wild type group, ##P < 0.01 vs wild type group. (B, C) Trimethylation of H3K4 in wild-type, heterozygote and mutant mice. Two representatives of each group are shown. **P < 0.01 vs wild type group, ##P < 0.01 vs wild type group. (D) Hematoxylin and eosin (HE) staining and immunohistochemical staining of VEGF, CD31, CD34 and Ki67 expression in wild-type (left panel), heterozygote (middle panel) and mutant mice (right panel). Representative images are shown. Percentage positive cells for (E) VEGF and Ki67 and (F) percentage positive staining area for CD31 and CD34 in wild-type, heterozygote and mutant mice determined immunohistochemically. **P < 0.01 vs wild type group, ##P < 0.01 vs wild type group. Data are mean ± SEM of 3 independent experiments.