Figure 5

Promotion of tumorgenesis and angiogenesis by RBP2-induced VEGF expression in vitro . (A, B) Western blot analysis of RBP2 and VEGF protein levels in cancer cells treated with control and RBP2 siRNA or cotransfection of RBP2 siRNA and VEGF expression vector. **P < 0.01 vs control, ##P < 0.01 vs RBP2 siRNA + pEGEP. (C) Foci formation of human gastric cancer cells after treatment with control and RBP2 siRNA or cotransfection of RBP2 siRNA and VEGF expression vector. (D) The foci numbers of clones. **P < 0.01 vs control. #P < 0.05 vs RBP2 siRNA + pEGEP. (E) ELISA results for VEGF concentration in cell cultures treated with control vector and RBP2 expression vector. **P < 0.01. (F) Tube formation in HUVEC cells treated with the supernatants from the cells transfected with control vector and RBP2 expression vector. (G) Tube formation calculated as the percentage of cell surface area to the total surface area. The group incubated with the control cell cultures were set to 100%. **P < 0.01. Data are mean ± SEM of 3 independent experiments.