Figure 6
Hyaluronan breaks down collagen IV and increases the hydraulic conductivity of GBC cells in vivo. Pretreatment with hyaluronan promotes myxoma virus oncolysis in GBC cells and enhances the oncolytic effects of myxoma virus against GBC in vivo. A. The immunohistochemistry results showing collagen IV distribution in xenograft tumors pretreated with DV (control), HA, or HA + anti-CD44 mAb in GBC-SD and SGC-996 cells. B. The H-Scores of collagen IV expression in GBC-SD and SGC-996 xenograft tumors (*, P < 0.05 compared with the control group). C. The hydraulic conductivity of xenograft tumors pretreated with DV (control), HA, or HA + anti-CD44 mAb in GBC-SD and SGC-996 cells (*, P < 0.05 compared with the control group). D. Following the addition of DV, Myx + Rap, Myx + HA, or Myx + HA + anti-CD44 mAb, frozen sections of the GBC-SD tumors from GBC-SD tumor-bearing mice were stained with DAPI and examined under fluorescence microscopy. E. Representative photographs of GFP-labeled virus in the tumors of GBC-SD tumor-bearing mice. F, G. In vivo treatment of GBC-SD and SGC-996 xenograft tumors with control DV, HA, Rap, Mvx, Myx + Rap, Myx + HA, and Myx + HA + anti-CD44 mAb. Tumor areas were evaluated over time by caliper measurement (*, P < 0.05 compared to Myx + Rap). H, I. Kaplan-Meier survival analysis of xenograft mice implanted with GBC-SD and SGC-996 after treatment with DV, HA, Rap, Myx, Myx + Rap, Myx + HA, and Myx + HA + anti-CD44 mAb (comparing the Myx + HA group with the Myx + Rap group, P = 0.002 and 0.002 in GBC-SD and SGC-996, respectively).