Figure 3
From: Effects of AKT inhibitor therapy in response and resistance to BRAF inhibition in melanoma
Basal levels of p-AKT and PTEN status in cell lines with differential sensitivity to dabrafenib and AKTi. Phosphorylation of AKT and PTEN status was determined by western blot analysis in a panel of cell lines with sensitivity or resistance to AKTi (A + B) or dabrafenib (C + D). Quantitation of p-AKT was done by using image quant software. Total AKT and GAPDH served as loading controls.