Figure 5

Combined treatment with dabrafenib and AKTi increases the subG1 fraction and induces apoptosis in cell lines sensitive to AKTi as single agent. Four representative melanoma cell lines with differential sensitivity to single agent AKTi and dabrafenib (Table 2) were cultured in DMSO (control), 1 μM staurosporine (positive control for apoptosis), 50 nM dabrafenib, 2.5 μM AKTi or the combination for 48 hours and stained with DAPI for cell cycle analysis (A) and anti-cleaved-PARP for apoptosis analysis (B). Bars represent the average cell fractions of minimum two independent experiments and the error bars represent the SEM. * p < 0.05 when comparing single agent dabrafenib with the combination treatment.