Figure 1

Expression of ICAM-1 is highly inversely correlated with E2F1 expression. A. ICAM-1 expression in DU145 cells. Whole cell lysates or total RNA were prepared from DU145 cells stably transfected with either the control vector or shRNA targeting E2F1. The expression of E2F1 and ICAM-1 was monitored by RT-PCR (upper) and Western blot (lower). B. ICAM-1 expression in DU145 cells was analyzed by real-time PCR. The stars indicate the significant differences (P < 0.05). C. E2F1 overexpression plasmids or control were transient transfected to DU145 cells in a 6-well plate for 48 h. The expression of E2F1 and ICAM-1 were measured by RT-PCR. D. The expression of E2F1 and ICAM-1 was measured by real time PCR. The stars indicate the significant differences (P < 0.05). E. Membrane expression of ICAM-1 in DU145/sh-E2F1 and control cells was measured by FACS. F. siRNA of E2F1 or scramble control was transient transfected to PC3 cells in a 6-well plate for 48 h. The expression of E2F1 and ICAM-1 in these cells were measured by RT-PCR. G. Forty pmol duplex siRNA of E2F1 or scramble control was transient transfected to Hela cells in a 6-well plate for 48 h. The expression of E2F1 and ICAM-1 in these cells were measured by RT-PCR (upper) and Western blotting analysis (lower).