Figure 1From: Targeted delivery of CD40L promotes restricted activation of antigen-presenting cells and induction of cancer cell deathDomain architecture and affinity purification of a scFv:CD40L fusion proteins. A ScFv:CD40L fusion proteins comprise a single chain variable fragment (scFv) of the specificity of interest genetically linked to the extracellular domain of CD40L (amino acids 116–261) and a FLAG-tag for affinity purification. Following production in eukaryotic cells, scFv:CD40L fusion proteins form stable homotrimeric fusion proteins. B The purity of a scFv:CD40L fusion protein isolated by affinity chromatography was assessed by gel electrophoresis and silver staining.Back to article page