Figure 6

miR-181a levels are inversely correlated with WIF-1 expression in CRC tissues. (A) The relationship between miR-181a expression and WIF-1 mRNA levels in CRC samples (90 cases). Statistically significant reverse correlations were observed between miR-181a and WIF-1 mRNA levels with the use of two-tailed Spearman’s test; (B) Western blot results show that the proteins expression level of WIF-1 is lower in tumor tissue (T ) than normal adjacent tissues (N). (C) The levels of miR-181a in above 8 pairs of sample tissues. (D) Expression patterns of WIF-1 immunohistochemistry in tissue microarrays of Cohort 2. The expression of WIF1 proteins in adjacent non-malignant mucosa (A), and CRC tissues with low (B) and high (C) WIF1 protein expression. Positive cells are stained brown. (E) Association between WIF1 expression in tumors and overall survival in 294 patients with CRC using immunohistochemistry (cohort 2). Kaplan-Meier overall survival curves for CRC patients with follow-up information were compared according to WIF1 expression level. Patients with lower WIF1 expression displayed a shorter overall survival after surgery.