Table 2 Primers for PCR and sequencing of PLK1
From: A 3′UTR polymorphism modulates mRNA stability of the oncogene and drug target Polo-like Kinase 1
Region | Sense (5′ – 3′) | Antisense (5′ – 3′) | Size (bp) |
|---|---|---|---|
Enhancer | GAGCAAGACTCCATCTCAACA | AACCAGGTGTAAGCCTCCCA | 590 |
Silencer | CTTGTATACAACATTGCACATGG | TCCTTCACCTGCCTTGCAGC | 603 |
Promoter | GCACTGCTCTGGGAGCTTGG | TGATGCAACGAAGCTGTCTGG | 820 |
Core Pro. | TCTTCCAACCTTCCCTCCCTC | GTCACTGCAGCACTCATGCTC | 690 |
Exon1 | GAGCGGTGCGGAGGCTCTGC | CAGGGCTTTCCTCCTCTTGTGC | 1240 |
Exon2 | GCTGTGCTGGAGAAGGAATG | ACAACCCACAAGTCAGTATCTTG | 455 |
Exon3 | CCTGGTTCTGGATGGTCAAA | ATTGTCATATCTTTCCCTGTCAC | 409 |
Exon4 | CTGCTCAGTGGTCTTAGGGATT | TATCCCACCTCTAAGGTAGCC | 359 |
Exon5 | AGTGGAGAACTTGGCATTG | CTCTGTCCTTCAATCCGTG | 513 |
Exon6 | TCCCCAAAGCAGTGGTAGC | TGTCTGCATAGGACCATTGGT | 385 |
Exon7 | CCCTGCTTTGCTCTTCTC | GTTACAGACTCTGGCCTTTTTGAGC | 322 |
Exon8 + 9 | CTGGGCTCAAACAATCCTCCTCCCTCA | GTGGGTTGAATGTGGAGTGAGCGGCT | 645 |
Exon10 | TCTTCCCTCTACTCCCTAACA | GGGTTCTACAGCCTTGTCC | 473 |