WNT5A siRNA decreases IL-6 and VEGF secretion in cell culture supernatants. The efficiency of WNT5A siRNA transfection was determined using Western blot (A) and RT-QPCR (B) 48 h and 72 h after transfection. Anti-β-actin was used as loading control. Cell culture supernatants were analyzed for differences in IL-6 protein (C) and VEGF protein using Elisa (E). The experiment was performed at least 3 times. Error bars represent SEM. Differences in IL-6 (D) and VEGF (F) mRNA from the same experiments were analyzed using RT-QPCR. The experiment was performed at least 3 times. The bar graphs show average fold decrease as compared to scramble siRNA (ctrl) for each time point indicated. Error bars represent SD. *** = p <0.001, ** = p <0.01 by ANOVA test.