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Figure 5 | Molecular Cancer

Figure 5

From: TFAP2B overexpression contributes to tumor growth and a poor prognosis of human lung adenocarcinoma through modulation of ERK and VEGF/PEDF signaling

Figure 5

TFAP2B knockdown inhibits VEGF/PEDF signaling and angiogenesis. Human H1299 cells were transfected with DC-based TFAP2B siRNA (si-TFAP2B) or overexpression plasmid (p-TFAP2B) nanoparticles. At 48 hours after transfection, the effects of TFAP2B knockdown or overexpression on the expression of TFAP2B, VEGF and PEDF at protein levels (A, B) and mRNA levels (D) were detected by Western blotting and RT-PCR, respectively. The amount of VEGF and PEDF proteins released in cell to the culture media were determined by ELISA, and the ratio of VEGF to PEDF in the media was also analyzed (C). Human umbilical vein endothelial cells (HUVEC) were transfected with DC-based TFAP2B siRNA (si-TFAP2B) nanoparticles. At 72 hours after transfection, the effect of TFAP2B knockdown on tube formation assay was analyzed (E). The data are presented as the mean ± SD of three separate experiments. *P < 0.05, significant differences between the treatment groups and control groups.

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Molecular Cancer

ISSN: 1476-4598