Figure 1
From: Long-term reduction of T-cell intracellular antigens leads to increased beta-actin expression
Stable TIA knockdown leads to an increased expression of beta-actin protein. (A) Exponentially growing HeLa cells stably transfected with control, TIA1, TIAR, TIA1 plus TIAR (1 + R) and HuR shRNAs were stained with anti-β-actin and anti-α-tubulin antibodies as well as incubated with phalloidin-TRITC and TO-PRO-3 reagents. The post-stained HeLa cells were visualized by fluorescence microscopy. (B) The fluorescence intensity of β-actin, phalloidin and α-tubulin were quantified from at least 30 different images per sample using ImageJ software. These quantifications are represented by histograms where the white, lightgray, darkgray, black and gray bars correspond to the control, TIA1, TIAR, TIA1 plus TIAR (1 + R) and HuR samples, respectively. The represented values were normalized and expressed relative to control (c), whose value is fixed arbitrarily to 1, and are means ± SEM (n = 30; *P < 0.05; **P < 0.01). The scale bar shows 8 μm.