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Table 1 Aur A/B and BRCA1/2 mediate cell polyploidy

From: The negative interplay between Aurora A/B and BRCA1/2 controls cancer cell growth and tumorigenesis via distinct regulation of cell cycle progression, cytokinesis, and tetraploidy

 

Cell lines1

Diploidy cells (%)

Tetraploidy cells (%)

N2

Capan-1

Scr

78.6 ± 3.2

16.2 ± 3.3

302

 

Aur Ai

82.3 ± 3.9 (*S↑)

11.6 ± 5.1 (*S↓)

300

 

Aur Bi

87.3 ± 5.9 (*S↑)

10.6 ± 4.6 (*S↓)

302

 

BRCA1i

72.8 ± 6.1 (*S↓)

22.3 ± 6.4 (*S↑)

303

 

Aur Ai-Aur Bi

89.0 ± 4.7 (*S↑)

6.4 ± 3.9 (*S↓)

306

 

Aur Bi-BRCA1i

83.4 ± 5.0 (*S↑)

9.3 ± 6.7 (*S↓)

303

 

Aur Ai-Aur Bi-BRCA1i

78.0 ± 4.8 (*AB↓)

10.0 ± 4.7 (*AB↑)

299

OVCA433

Scr

88.9 ± 4.5

11.1 ± 4.5

287

 

Aur Ai

95.0 ± 5.0 (*S↑)

5.0 ± 5.0 (*S↓)

298

 

Aur Bi

95.0 ± 2.1 (*S↑)

5.0 ± 2.1 (*S↓)

302

 

BRCA1i

80.0 ± 6.3 (*S↓)

20.0 ± 6.3 (*S↑)

295

 

Aur Ai-Aur Bi

97.8 ± 2.4 (*S↑)

2.2 ± 2.4 (*S↓)

300

 

BRCA1i-BRCA2i

77.8 ± 6.1 (*S↓)

22.2 ± 6.1 (*S↑)

290

 

BRCA1i-BRCA2i-Aur Ai

90.0 ± 4.2 (*B1B2↑)

10.0 ± 4.2 (*B1B2↓)

294

 

BRCA1i-BRCA2i-Aur Bi

84.6 ± 4.7 (*B1B2↑)

14.4 ± 4.9 (*B1B2↓)

290

 

BRCA1i-BRCA2i-Aur Ai-Aur Bi

91.2 ± 4.8 (*B1B2↑)

7.8 ± 5.6 (*B1B2↓)

290

  1. 1For each cell line, 30–36 cells in metaphase in every slide were examined. The assays were repeated three times. The increase or decrease of cells with diploidy or tetraploidy was indicated as “↑” or “↓”, respectively. *p < 0.05. S, AB or B1B2 represent cells expressing Scr, Aur Ai-Aur Bi or BRCA1i-BRCA2i used for statistical analysis.
  2. 2 N represents the total number (N) of cells karyotyped for each cell line.